Compared to the control group, the percentages of these lymphocyte subsets expressing TLR2 also appeared higher in the NPGN group, but only the difference in the frequency of CD4+TLR-2+ cells was significant (= 0

Compared to the control group, the percentages of these lymphocyte subsets expressing TLR2 also appeared higher in the NPGN group, but only the difference in the frequency of CD4+TLR-2+ cells was significant (= 0.003, Table Diclofenac diethylamine 3). 2.4. concentration was nearly 37% over the normal range. Urea and BUN concentrations in the PGN group were also higher than in the control group, but the differences were not statistically significant (Table 1). Both groups of patients experienced a significantly lower IgG concentration compared to control, with the concentration in NPGN patients being lower than in PGN patients (= 0.068). NPGN patients experienced an IgA concentration that was statistically lower than that of PGN patients (= 0.025), but it was not significantly different from that of the controls (= 0.431)). Regarding the total concentration of protein in serum, and Rabbit Polyclonal to Sodium Channel-pan the concentration of albumin, both groups of patients experienced a significantly lower value than the control group ( 0.001). Both groups of patients had a significantly higher quantity of protein after a 24-h urine collection test compared to control, with the concentration in the NPGN group being higher than in the PGN group (= 0.072; Table 1). Patients in the PGN group experienced a significantly higher hemoglobin concentration compared to the control group (= 0.022; Table 2). 2.3. Comparisons of TLR2 Expression on T and B Lymphocyte Subsets Diclofenac diethylamine between Patients with PGN, NPGN, and Controls The percentages of CD4+ T cells, CD8+ T cells, and CD19+ B cells expressing TLR2 in the PGN group were significantly higher than those in the NPGN group and the control group ( 0.001; Table 3). Compared to the control group, the percentages of these lymphocyte subsets expressing TLR2 also appeared higher in the NPGN group, but only the difference in the frequency of CD4+TLR-2+ cells was significant (= 0.003, Table 3). 2.4. Correlations between TLR2 Expression on T and B Lymphocyte Subsets and Determined Laboratory Parameters in NPGN and PGN Patients Next, we investigated the relationship between TLR2 expression and the laboratory parameters that showed statistical differences (i.e., urine protein excretion and the serum concentrations of urea, BUN, IgG, IgA, total protein, and albumin). In patients with PGN, the Diclofenac diethylamine frequencies of CD4+TLR-2+ cells and CD19+TLR-2+ cells correlated negatively with the concentration of IgG (rho = ?0.579, Diclofenac diethylamine = 0.026; rho = ?0.561, = 0.032, respectively; Physique 1). Moreover, the frequency of CD4+TLR-2+ cells in patients Diclofenac diethylamine with PGN correlated negatively with the concentration of albumin (rho = ?0.631, p=0.012; Physique 1) and positively with urinary protein excretion (rho = 0.636, = 0.013). The remaining correlations were not significant. Table 4 shows all Spearman correlation coefficients. Open in a separate window Physique 1 Correlation between the expression of TLR-2 and selected laboratory measures in patients with PGN. R: Spearman correlation coefficient. (A). Correlation between the frequencies of CD4+TLR-2+ cells and the concentration of IgG (rho = ?0.58, = 0.026). (B). Correlation between the frequencies of CD19+TLR-2+ cells and the concentration of IgG (rho = ?0.56, = 0.032). (C). Correlation between the frequencies of CD4+TLR-2+ cells and the concentration of albumin (rho = ?0.63, = 0.012). (D). Correlation between the frequencies of CD4+TLR-2+ cells and the urinary protein excretion (rho = 0.64, = 0.013). Table 4 Correlations between the frequency of TLR-2-positive lymphocyte subpopulations and selected laboratory variables in patients with proliferative glomerulonephritis (PGN) and non-proliferative glomerulonephritis (NPGN). = 0.7170.05= 0.863?0.25,= 0.258?0.15= 0.584?0.12, = 0.589?0.17= 0.549 BUN (mg/dL) ?0.08,= 0.980?0.58= 0.026?0.28,=.