The flowthrough containing BbKI was concentrated and additional purified by size-exclusion chromatography (Superdex 75 HR 10/30 column; GE Health care) in 20?mTrisCHCl pH 7
The flowthrough containing BbKI was concentrated and additional purified by size-exclusion chromatography (Superdex 75 HR 10/30 column; GE Health care) in 20?mTrisCHCl pH 7.5, 0.2?NaCl. To...